[Oral Session] Genetic engineering

○ Denotes Presenter.

  • 13:54　PC Checking time

Moderators: Susumu Uchiyama, Minetaka Sugiyama

  • 14:06 (2Gp01)
    PER1, GUP1 and CWH43 of methylotrophic yeast Ogataea minuta are involved in cell wall integrity
    ……Xin-Xin Xu1,2, Akiko Komatsuzaki1, Yasunori Chiba1, Xiao-Dong Gao2, ○Takehiko Yoko-o1 (1BRD, AIST,2Jiangnan Univ. (China))
     
  • 14:18 (2Gp02)
    Polyploid generation by multi-step mating of Saccharomyces cerevisiae
    ……○Nobuo Fukuda, Shinya Honda (Biomed. Res. Inst., AIST)
     
  • 14:30 (2Gp03)
    Mutagenesis and selection of carotenoid hyper-producing mutants using DNA-cleaving antibiotics
    ……○Yusuke Watabe, Katsumasa Abe, Yosio Kera, shouji Takahashi (Nagaoka Univ. Technol.)
     
  • 14:42 (2Gp04)
    Analysis for surviving cells appeared with induction of excision of centromeric DNA from a chromosome in Saccharomyces cerevisiae
    ……○Hiroaki Matsuzaki, Yuki Fujiwara, Soichiro Hirashima, Akihiro Miyamoto, Toshiaki Yanamoto, Takushi Hatano (Fac. Life Sci. Biotechnol., Fukuyama Univ.)
     
  • 14:54 (2Gp05)
    Construction of a strain library with genomic diversities by the novel chromosome engineering technology and its application for strain breeding in the budding yeast
    ……○Yu Sasano, Soichiro Ikura, Yukiko Takaki, Kazuma Takata, Hiromichi Ideguchi, Hisataka Taguchi (Fac. Biotechnol. Life Sci., Sojo Univ.)
     
  • 15:06 (2Gp06)
    Efficient homologous recombination in basidiomycetous yeast Pseudozyma antarctica by CRISPR/Cas9
    ……○Takumi Tanaka1, Emi Kunitake1,2, Tohru Yarimizu1, Akira Endoh1, Etsuko Katoh1, Hiroko Kitamoto1 (1NARO,2Grad. Sch. Bioresour., Mie Univ.)
     
  • 15:18　PC Checking time

Moderators: Takehiko Yoko-o, Yu Sasano

  • 15:30 (2Gp07)
    Isolation of the effective promotor sequences for recombinant gene expression in basidiomycetous yeast Pseudozyma antarctica
    ……○Tohru Yarimizu1, Koike Hideaki2, Tomotake Morita3, Takumi Tanaka1, Hiroko Kitamoto1 (1Institute for Agro-Environmental Sciences.,2BRI. AIST,3AIST)
     
  • 15:42 (2Gp08)
    Functional analysis of Pichia Kudriavzevii N77-4 isolated from the Korean traditional fermentation starter Nuruk
    ……○Minetaka Sugiyama1, Aoba Matsushita2, Shohei Takashima2, Natsumi Miyashita2, Ryota Murayama1, Kei Ishida1, Takeru Fukaya1, Susumu Uchiyama1, Seong Yeol Baek3, Ji-Young Mun3, Soyoung Kim3, Soo-Hwan Yeo3 (1Grad. Sch. Eng., Osaka Univ.,2Faculty of Eng., Osaka Univ.,3Dept. Agrofood Resources・NIAS・RDA)
     
  • 15:54 (2Gp09)
    Deletion of DNA ligase IV homolog confers higher gene targeting efficiency on homologous recombination in Pichia pastoris
    ……○Yoichiro Ito1, Toru Watanabe2, Shimpei Aikawa1, Teruyuki Nishi2, Tozo Nishiyama2, Yasuyuki Nakamura1, Tomohisa Hasunuma1, Yoshihiko Yasohara2, Jun Ishii1, Akihiko Kondo1 (1Grad. Sch. Sci. Technol. Innov., Kobe Univ.,2Kaneka Corp.)
     
  • 16:06 (2Gp10)
    Construction of a new stable, autonomously replicating plasmid vector containing Pichia pastoris centromeric DNA
    ……○Yasuyuki Nakamura1, Teruyuki Nishi1,2, Risa Noguchi3, Yoichiro Ito1, Toru Watanabe2, Tozo Nishiyama2, Shimpei Aikawa1, Tomohisa Hasunuma1, Jun Ishii1, Yoshihiko Yasohara2, Akihiko Kondo1 (1Grad. Sch. Sci. Technol. Innov., Kobe Univ.,2Kaneka Corp.,3TRAHED)
     
  • 16:18 (2Gp11)
    A simple and efficient DNA multi-assemble method using episomal vector in Pichia pastoris
    ……○Teruyuki Nishi1,2, Tozo Nishiyama2, Taiki Yamaji3, Masaya Tamai3, Toru Watanabe2, Yasuyuki Nakamura1, Yoichiro Ito1, Jun Ishii1, Akihiko Kondo1, Yoshihiko Yasohara2 (1Grad. Sch. Sci. Technol. Innov., Kobe Univ.,2Kaneka Corp.,3TRAHED)
     

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