Genetic engineering (3Ba01–3Ba11)

○ Denotes Presenter.

  • 8:48 PC Check (12 min)

Moderators: Hisashi Hoshida, Takehiko Yoko-o

  • 9:00 (3Ba01)
    Characterization of gene expression of AOX1, which encodes alcohol oxidase, in methylotrophic yeast Ogataea minuta
    …… ○Takehiko Yoko-o1, Erina Yoshihara1,2, Akiko Komatsuzaki1, Mariko Umemura2, Yasunori Chiba1 (1BRD, AIST,2Sch.Life Sci., Tokyo Univ. Pharm. Life Sci.)
     
  • 9:12 (3Ba02)
    Effect of deltarhodopsin expression on the yeast metabolism
    …… ○Kensuke Otsuka1, Yoko Hirono2, Hiroko Ishii3, Jun Ishii3, Nobuyuki Okahashi1, Yoshihiro Toya1, Kiyotaka Hara2, Fumio Matsuda1 (1Grad. Sch. IST, Osaka Univ.,2Sch. Food Nutr. Sci., Univ. Shizuoka.,3Grad. Sch. Sci. Technol. Innov., Kobe Univ.)
     
  • 9:24 (3Ba03)
    Explore the tolerant mechanism of Pichia membranifaciens KS47-1 of inhibitor tolerant yeast by RNA-seq
    …… ○Kento Takeuchi1, Yuta Kato1, Tomoko Arakawa1, Jun-ichi Horiuchi2, Masaaki Konishi3 (1Grad. Sch. Bio. Environ., Kitami Inst Tech,2Dept. Biomole. Eng. Kyoto Inst. Tech.,3Sch. Reg. Innov. Soc. Design Eng. Kitami Inst. Tech.)
     
  • 9:36 (3Ba04)
    Single amino acid substitutions of secretory signal MFα make large improvement for secretion of recombinant proteins in Pichia pastoris
    …… ○Yoichiro Ito1, Misa Ishigami2, Noriko Hashiba2, Yasuyuki Nakamura1, Tomohisa Hasunuma1, Jun Ishii1, Akihiko Kondo1 (1Grad. Sch. Sci. Technol. Innov., Kobe Univ.,2TRAHED)
     
  • 9:48 (3Ba05)
    A specific region of a lucferae coding sequence interferes with its expression and is involved in intron-mediated enhancement
    …… ○Hiroki Kikuta1, Hisashi Hosida1,2,3, Rinji Akada1,2,3 (1Dept. Appli. Chem., Grad. Sch. Sci. Tech. Innov., YamaguchiUniv.,2YURC-TMR.,3YUBEC)
     
  • 10:00 (3Ba06)
    Effects of mutations introduced through adaptive laboratory evolution on glycerol assimilation ability in Saccharomyces cerevisiae
    …… ○Masato Sone1, Shunsuke Takahashi1, Kazuki Kawai1, Chikara Furusawa2,3, Takashi Hirasawa1 (1Sch. Life Sci. Technol, Tokyo Tech,2BDR, RIKEN,3UBI, Univ. Tokyo)
     
  • 10:12 Break & PC Check (12 min)

Moderators: Jun Ishii, Minetaka Sugiyama

  • 10:24 (3Ba07)
    Histone gene analysis with a plasmid that can be used for gene expression in yeast and human cluture cells
    …… ○Takahiro Ono1, Hisashi Hoshida1,2,3, Rinji Akada1,2,3 (1Dept. Appli. Chem., Grad. Sch. Sci. Tech. Innov., YamaguchiUniv.,2YURC-TMR.,3YUBEC)
     
  • 10:36 (3Ba08)
    Mechanism of adaptation to high temperature stress in Saccharomyces cerevisiae SPY3
    …… Hinako Isozaki1, Masumi Yamasaki1, Shun Sawada1, Daiki Kudo1, Allad Devanadera2, Ivy Grace Pait2, Irene Pajares2, Fidel Rey P. Nayve Jr.2, ○Minetaka Sugiyama1 (1Grad. Sch. Eng., Osaka Univ.,2BIOTECH, UPLB)
     
  • 10:48 (3Ba09)
    Extrachromosomal circularization of targeted DNA region in the budding yeast
    …… ○souichiro Ikuta, Hisatomi Ayumu, Yu Sasano, Hisataka Taguchi (Grad. Sch. Eng., Sojo Univ.)
     
  • 11:00 (3Ba10)
    Cloning and expressing recombinant glutamate decarboxylase (GAD) for boost of GABA production in beer-producing yeast
    …… ○Pik Kuan Low, John Chi-Wei Lan (Biorefin. Bioprocess Eng. Lab., Dept. Chem. Eng. Mater. Sci., Coll. Eng., Yuan Ze Univ., Taiwan 32003, R.O.C.)
     
  • 11:12 (3Ba11)
    Development of yeast genetic switches using a novel tri-functional fusion protein consisting of positive/negative selection markers and fluorescent protein
    …… ○Kenta Nozaki1, Masahiro Tominaga1, Daisuke Umeno2, Akihiko Kondo1,3, Jun Ishii1,3 (1Grad. Sch. Sci. Technol. Innov., Kobe Univ.,2Grad. Sch. Eng., Chiba Univ.,3Eng. Biol. Res. Ctr., Kobe Univ.)

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