Enzymology, enzyme (2Ap01–2Ap18)

○ Denotes Presenter.

  • 13:18　PC Check (12 min)

Moderators: Yuichi Koga, Mamoru Wakayama

  • 13:30 (2Ap01)
    Quick and simple purification and structural analysis of recombinant L-methionine decarboxylase.
    …… ○Atsushi Okawa1*, Masaya Hayashi1, Masaki Murota2, Tomoo shiba2, Shigeharu Harada2, Michio Nemoto1, Takashi Tamura1, Kenji Inagaki1 (1Grad. Sch. Environ. Life Sci., Okayama Univ.,2Grad. Sch. Sci. Technol., Kyoto Inst. Technol.)
    *Winner of the 2019 SBJ Excellent Student Award (Hisho Award)  
     
  • 13:42 (2Ap02)
    Functional analysis of active site residues of L-glutamate oxidase
    …… ○Yoshika Yano1, Shinsaku Matsuo1, Nanako Itou2, Katsumi Imada2, Hitoshi Kusakabe3, Michiko Nemoto1, Takashi Tamura1, Kenji Inagaki1 (1Grad. Sch. Environ. Life Sci., Okayama Univ.,2Grad. Sch. Sci., Osaka Univ.,3Enzyme Sensor Co. Ltd.)
     
  • 13:54 (2Ap03)
    Improvement of substrate inhibition and substrate specificity of glycine oxidase from Geobacillus kaustophilus
    …… ○Yuki Higashiura1, Ryusuke Oda1, Taishi Kawasaki1, Takaomi Nomura2, Ryoichi Arai2, Yoshiaki Nishiya1 (1Dept. Life Sci., Setsunan Univ. Sci. Eng.,2Dept. Appl. Biol., FTST, Shinshu Univ.)
     
  • 14:06 (2Ap04)
    Enzymatic glycine analysis method using a stable glycine oxidase mutant
    …… ○Moemi Tatsumi1, Wataru Hoshino1, Yuya Kodama1, Techawaree Ueatrongchit2, Kazutoshi Takahashi1, Hiroki Yamaguchi1, Uno Tagami1, Hiroshi Miyano1, Yasuhisa Asano2, Toshimi Mizukoshi1 (1Ajinomoto Co., Inc.,2Biotechnol. Res. Center, Toyama Pref. Univ.)
     
  • 14:18 (2Ap05)
    The effect of propeptide binding affinity on maturation process of the protease from a hyperthermophilic archaeon
    …… ○Nagao Masahide, Maegawa Daiki, Yamano-Adachi Noriko, Koga Yuichi, Omasa Takeshi (Grad. Sch. Eng., Osaka Univ.)
     
  • 14:30 (2Ap06)
    Studies on reconstruction of a thermostable protease using protein trans-splicing
    …… ○Yusuke Tatsumi, Noriko Yamano-Adachi, Yuichi Koga, Takeshi Omasa (Grad. Sch. Eng., Osaka Univ.)
  • 14:42 Break & PC Check (12 min)

Moderators: Jiro Arima, Shin Suzuki

  • 14:54 (2Ap07)
    High expression system of recombinant L-amino acid esterase from Elizabethkingia sp. TT1 and Val-Gly synthesis in cell reaction
    …… ○Atsuya Onishi1, Takahiro Tanaka1, Junji Hayashi2, Yosuke Toyotake1, Mamoru Wakayama1 (1Grad. Sch. Life Sci., Ritsumeikan Univ.,2Grad. Sch. Biosci. Bioind, Tokushima Univ.)
     
  • 15:06 (2Ap08)
    Synthesis of β-aspartyl compound using asparagine synthetase
    …… ○Taizo Yamada1, Junji Hayashi2, Yosuke Toyotake1, Mamoru Wakayama1 (1Grad. Sch. Life Sci., Ritsumeikan Univ.,2Grad. Sch. Bioresource, Tokushima Univ.)
     
  • 15:18 (2Ap09)
    Cloning, expression and characterization of β-aspartyl transpeptidase gene from Thermococcus kodakarensis
    …… ○Aoi Fukase1, Junji Hayashi2, Kazuyoshi Takagi1, Yousuke Toyotake1, Mamoru Wakayama1 (1Grad. Sch. Life Sci., Ritsumeikan Univ.,2Grad. Sch. Biosci. Bioind, Tokushima Univ.)
     
  • 15:30 (2Ap10)
    Effect on activity and stability of γ-glutamyl transpeptidase II C-terminal region from Pseudomonas aeruginosa PAO1 strain
    …… ○Seiji Hatta1, Yuki Nonomura1, Junnji Hayashi2, Kazuyoshi Takagi1, Yosuke Toyotake1, Mamoru Wakayama1 (1Grad. Sch. Life Sci., Ritsumeikan Univ.,2Grad. Sch. Adv. Technol. Sci., Tokushima Univ.)
     
  • 15:42 (2Ap11)
    Studies on structure and function of the γ-glutamyltranspeptidase from Pseudomonas nitroreducens
    …… ○Hiroki Ikezoe1, Junji Hayashi2, Yosuke Toyotake1, Mamoru Wakayama1 (1Grad. Sch. Life Sci., Ritsumeikan Univ.,2Fac. Bioresource, Tokushima Univ.)
     
  • 15:54 (2Ap12)
    Immobilization and site directed mutagenesis of Trp525 residue of γ-Glutamyltranspeptidase from Pseudomonas nitroreducens (PnGGT) to improve Theanine production
    …… ○Putthapong Phumsombat1, Chiharu Sano1, Takao Hibi2, Takafumi Itoh2, Junji Hayashi1, Yosuke Toyotake1, Mamoru Wakayama1 (1Coll. Life Sci., Ritsumeikan Univ.,2Dept. Biosci. Fukui Prefectural Univ.)
     
  • 16:06　Break & PC Check (12 min)

Moderators: Takashi Tamura, Hirotoshi Matsumura

  • 16:18 (2Ap13)
    Discovery of pyrazine synthesis enzymes originating from L-amino acids and its analysis
    …… ○Tomoharu Motoyama1,2*, Shogo Nakano1, Yuta Yamamoto3, Hiroaki Tokiwa3, Yasuhisa Asano4, Sohei Ito1 (1Grad. Sch. Integr. Pharm. Nutr. Sci., Univ. Shizuoka,2JSPS,3Rikkyo Univ.,4Fac. Eng., Toyama Pref. Univ.)
    *Winner of the 2019 SBJ Excellent Student Award (Hisho Award)  
     
  • 16:30 (2Ap14)
    Development of bioprocess for practical trans-3-hydroxyproline production
    …… ○Ryotaro Hara1,4, Takeyuki Nishikawa2, Takuya Okuhara2, Kento Koketsu3, Kuniki Kino1,2 (1Res. Inst. Sci. Eng., Waseda Univ.,2Sch. Adv. Sci. Eng., Waseda Univ.,3KYOWA HAKKO BIO CO., LTD.,4Grad. Sch. Agric., Kyoto Univ.)
     
  • 16:42 (2Ap15)
    Development of an enzymatic method for production of D-amino acid-containing dipeptides
    …… ○Tomoya Katsume, Shin Suzuki, Kuniki Kino (Dept. Appl. Chem., Sch. Adv. Sci. Eng., Waseda Univ.)
     
  • 16:54 (2Ap16)
    Synthesis of ferulic acid amides by a novel chemoenzymatic process
    …… ○Shin Suzuki, Yuri Hayata, Kuniki Kino (Dept. Appl. Chem., Sch. Adv. Sci. Eng., Waseda Univ.)
     
  • 17:06 (2Ap17)
    Characterization of L-amino acid deaminase from Proteus vulgaris and application to ammonia production
    …… ○Sayaka Miyazawa, Satoru Umezawa, Kuniki Kino (Dept. Appl. Chem., Grad. Sch. Adv. Sci. Eng., Waseda Univ.)
     
  • 17:18 (2Ap18)
    Recombinant expression, purification, and biochemical and structural charactrization of novel nitrilase degrading cyanide ion.
    …… ○Haruya Sasaki1, Takahiro Ogawa2, Daisuke Komatu2,3, Natsumi Kagaya4, Hirotoshi Matumura1, Masafumi Odaka1, Masafumi Yohda2 (1Grad. Sch. Eng., Sci., Akita Univ.,2Grad. Sch. Eng., Tokyo Univ. Agric. Technol.,3IS-solution Co.,,4Faculty of. Sci. and Eng., Akita Univ.)

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