Journal of Bioscience and Bioengineering Vol. 99, No. 2 (2005)
Vol. 99, February 2005
Functionalization of the cytochrome P450cam monooxygenase system.
Functionalization of the cytochrome P450cam monooxygenase system, which requires electron transfer among three different proteins, was successfully attained in the micro-scale aqueous compartments of stable water-in-oil (W/O) emulsions, which can be easily formed by mixing organic and aqueous solutions. The activity of the cytochrome P450 monooxygenase system as to hydroxylation of camphor, as a model, was efficiently improved through coupling of the system with the NADH regeneration process, as illustrated on the cover of this issue, suggesting the potential utility of the micro-scale cell-like aqueous compartments of W/O emulsions for practical multicomponent enzymatic reactions, especially for substrates with low aqueous solubility.
Related article: Michizoe, J., Ichinose, H., Kamiya, N., Maruyama, T., and Goto, M., “Functionalization of the cytochrome P450cam monooxygenase system in the cell-like aqueous compartments of water-in-oil emulsions“, J. Biosci. Bioeng., vol. 99, 12-17 (2005).
⇒JBBアーカイブ：Vol.107 (2009) ～最新号
⇒JBBアーカイブ：Vol. 93（2002）～Vol. 106（2008）
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