Journal of Bioscience and Bioengineering Vol. 106, No. 6 (2008)
Vol. 106, December 2008
Optimization of the timing for effective gene transduction into the chicken gonads.
To determine the best timing of viral injection for the expression and transduction of a transgene in the gonads, a viral solution was injected into chicken embryos at various stages of development after incubation for 50–60 h (stages 14–17).
The left panel shows embryonic developmental stage at the time of viral injection which was determined by microscopically assessing their size and shape; upper left, stage 14; upper right, stage 15; lower left, stage 16; lower right, stage 17.
The right panel shows the expression of LacZ in gonads isolated from manipulated embryos (just before hatching) after injecting a retroviral vector carrying LacZ gene at stage 15; upper, testis (L); middle, testis (R); lower, ovary.
Related article: Kawabe, Y., Naka, T., Komatsu, H., Nishijima, K., Iijima, S., and Kamihira, M., "Retroviral gene transduction into chicken embryo gonads through blood circulation”, J. Biosci. Bioeng., Volume 106, Issue 6, Pages 598–601 (2008).
⇒JBB Archive Top
⇒JBB Archive: Vol. 93 (2002)–Vol. 106 (2008)
.jpg)